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Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with PE-Labeled Monoclonal Anti-G4S linker Antibody, Mouse IgG1 (AM635)(Cat. No. G4S-PFM635) at 1:50 dilution(2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) , compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Non-specificity of PE-Labeled Monoclonal Anti-G4S linker Antibody, Mouse IgG1 (AM635)(Cat. No. G4S-PFM635) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-G4S linker Antibody (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
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